Journal: Journal of Ovarian Research

Article Title: Obacunone alleviated the development of polycystic ovary syndrome via inhibiting STAT3 phosphorylation

doi: 10.1186/s13048-025-01934-6

Figure Lengend Snippet: Colivelin TFA reversed OB roles on apoptosis, inflammatory response and oxidative stress in DHEA-treated KGN cells. A Western blot for assessing p-STAT3 and STAT3 expression; ( B ) Flow cytometry was applied for detecting the apoptosis; ( C ) Western blot for assessing inflammatory factors expression; ( D ) DCFH-DA fluorescent probe for assessing ROS level; ( E ) The detection of MDA, GSH, SOD and CAT level. * P < 0.05, # P < 0.05, $ P < 0.05, & P < 0.05

Article Snippet: KGN cells were treated with 500 nM DHEA for 24 h, followed by removing the medium and replacing medium supplemented with OB (20, 40 and 80 μM) or Colivelin TFA (a STAT3 activator, 0.5 μM, MedChemExpress, USA) for two days [ – ].

Techniques: Western Blot, Expressing, Flow Cytometry

Journal: European Journal of Medical Research

Article Title: CLCF1 contributes to high-glucose-induced EndMT by modulating JAK2/STAT3 signaling

doi: 10.1186/s40001-025-02932-6

Figure Lengend Snippet: CLCF1 promotes HRGEC injury and EndMT under HG stimulation via JAK2/STAT3 signaling. A , B Differential expression of JAK2/STAT3 pathway components identified through transcriptomic sequencing. C qRT-PCR analysis of JAK2 and STAT3 expression across different groups, normalized to β-actin. D , E Representative western blot images and quantification of JAK2, STAT3, p-JAK2, and p-STAT3 protein expression levels in different groups (Quantitative data represent p-JAK2/JAK2 or p-STAT3/STAT3 ratios normalized to β-actin). * p < 0.05,** p < 0.01

Article Snippet: To determine whether JAK2/STAT3 pathway activation rescues the protective effects of CLCF1 knockdown, HG-exposed HRGECs transfected with CLCF1 siRNA were treated with the STAT3 agonist colivelin (1 μM, Selleck Chemicals) for 48 h (Fig. A).

Techniques: Quantitative Proteomics, Sequencing, Quantitative RT-PCR, Expressing, Western Blot

Journal: Journal of Nanobiotechnology

Article Title: Single-cell multi-omics analysis reveals the mechanism of action of a novel antioxidant polyphenol nanoparticle loaded with STAT3 agonist in mediating cardiomyocyte ferroptosis to ameliorate age-related heart failure

doi: 10.1186/s12951-025-03317-x

Figure Lengend Snippet: Impact of STAT3 Gene on the Biological Functions of CMs. A Schematic diagram of experimental procedure involving lentivirus transfection for silencing or overexpression of STAT3; B Viability changes of CMs in different intervention groups at 12, 24, 36, and 48 h detected by CCK-8 assay; C Assessment of proliferative capacity of CMs in different intervention groups using EDU experiment, with EDU-positive cells shown in red indicating cells in the proliferative phase, and blue representing DAPI-stained cell nuclei (scale bar = 50 μm); D Statistical analysis of EDU staining results; E Assessment of apoptosis in CMs in different intervention groups using Annexin V/PI double staining flow cytometry, with the bar graph showing the percentage of cells in Q2 and Q3 quadrants indicating apoptotic cells; F Evaluation of migration of CMs in different intervention groups using wound healing assay (scale bar = 100 μm); G Evaluation of migration capacity of CMs in different intervention groups using Transwell assay (scale bar = 50 μm). Quantitative data in the figures are presented as Mean ± SD. Each cell experiment was repeated 3 times. * denotes comparison between two groups, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Subsequently, 2 mg of the STAT3 agonist Colivelin (referred to as Col, HY-P1061, MedChemExpress, USA) was added to the mixture and vigorously stirred at 30 °C.

Techniques: Transfection, Over Expression, CCK-8 Assay, Staining, Double Staining, Flow Cytometry, Migration, Wound Healing Assay, Transwell Assay, Comparison

Journal: Journal of Nanobiotechnology

Article Title: Single-cell multi-omics analysis reveals the mechanism of action of a novel antioxidant polyphenol nanoparticle loaded with STAT3 agonist in mediating cardiomyocyte ferroptosis to ameliorate age-related heart failure

doi: 10.1186/s12951-025-03317-x

Figure Lengend Snippet: Influence of STAT3 on CMs Cell Ferroptosis. A Representative fluorescence images of CMs transfected with different lentiviruses for intracellular iron levels identified using FerroOrange (Scale bar = 100 μm); B Statistical analysis of iron content in CMs from different intervention groups using assay kits; C GSH/GSSG ratio in CMs from different intervention groups; D – E Visualization of ROS production in CMs using DCFH-DA under confocal laser scanning microscopy and statistical analysis using flow cytometry (Scale bar = 25 μm); F Measurement of MDA content in different intervention groups; G Representative confocal images of CMs stained with C11-BODIPY 581/591. Red indicates unoxidized lipids, while green represents oxidized lipids (Scale bar = 25 μm); H – I Transcription levels of ferroptosis-related genes SLC7A11 and GPX4 detected by RT-qPCR; J Observation of mitochondrial morphology in cells using TEM, with red arrows pointing to mitochondrial structures (scale bar = 1 μm); K Detection of mitochondrial membrane potential (MMP: mitochondrial membrane potential) in cells from different intervention groups using JC-1 assay. Quantitative data in the figures are presented as Mean ± SD. Each cell experiment was repeated 3 times. * denotes comparison between two groups, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Subsequently, 2 mg of the STAT3 agonist Colivelin (referred to as Col, HY-P1061, MedChemExpress, USA) was added to the mixture and vigorously stirred at 30 °C.

Techniques: Fluorescence, Transfection, Confocal Laser Scanning Microscopy, Flow Cytometry, Staining, Quantitative RT-PCR, Membrane, Comparison

Journal: Journal of Nanobiotechnology

Article Title: Single-cell multi-omics analysis reveals the mechanism of action of a novel antioxidant polyphenol nanoparticle loaded with STAT3 agonist in mediating cardiomyocyte ferroptosis to ameliorate age-related heart failure

doi: 10.1186/s12951-025-03317-x

Figure Lengend Snippet: Impact of PN@Col on the Biological Functions of CMs. A Expression changes of p-STAT3 protein in CMs cells from different treatment groups detected by Western blot; B Viability changes of CMs in different treatment groups at 12, 24, 36, and 48 h assessed by CCK-8 assay; C Evaluation of proliferative capacity of CMs in different treatment groups using EDU experiment, with red fluorescence indicating EDU-positive cells in the proliferative phase and blue fluorescence representing DAPI-stained cell nuclei (scale bar = 50 μm); D Assessment of cell migration in different intervention groups of CMs using wound healing assay (scale bar = 100 μm); E Evaluation of migration capacity of CMs in different treatment groups using Transwell assay (scale bar = 50 μm); F Detection of apoptosis in CMs from different treatment groups using Annexin V/PI double staining flow cytometry, with bar graph representing the percentage of cells in Q2 and Q3 quadrants indicating apoptotic cells; G Representative fluorescence images of intracellular iron levels in CMs from different treatment groups identified using FerroOrange (Scale bar = 25 μm); H Visualization of ROS production in CMs using DCFH-DA under confocal laser scanning microscopy and statistical analysis using flow cytometry (Scale bar = 25 μm); I Representative confocal images of CMs stained with C11-BODIPY 581/591. Red indicates unoxidized lipids, while green represents oxidized lipids (Scale bar = 25 μm); J GSH/GSSG ratio in CMs from different treatment groups; K Measurement of MDA content in CMs from different treatment groups; L – M Transcription levels of ferroptosis-related genes SLC7A11 and GPX4 detected by RT-qPCR; N Observation of mitochondrial morphology in cells using TEM, with red arrows indicating mitochondrial structures (scale bar = 1 μm); O Detection of mitochondrial membrane potential (MMP) in cells from different treatment groups using JC-1 assay. Quantitative data in the figures are presented as Mean ± SD. Each cell experiment was repeated 3 times. * denotes comparison between two groups, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: Subsequently, 2 mg of the STAT3 agonist Colivelin (referred to as Col, HY-P1061, MedChemExpress, USA) was added to the mixture and vigorously stirred at 30 °C.

Techniques: Expressing, Western Blot, CCK-8 Assay, Fluorescence, Staining, Migration, Wound Healing Assay, Transwell Assay, Double Staining, Flow Cytometry, Confocal Laser Scanning Microscopy, Quantitative RT-PCR, Membrane, Comparison

Journal: Journal of Nanobiotechnology

Article Title: Single-cell multi-omics analysis reveals the mechanism of action of a novel antioxidant polyphenol nanoparticle loaded with STAT3 agonist in mediating cardiomyocyte ferroptosis to ameliorate age-related heart failure

doi: 10.1186/s12951-025-03317-x

Figure Lengend Snippet: Impact of PN@Col on Age-related HF Mouse Cardiac Function. A Representative cardiac ultrasound images of mice in different treatment groups; B Representative left ventricular pressure waveforms, with a scale of 40 mmHg vertically and 100 ms horizontally; C Anatomical diagrams of mouse hearts from different treatment groups; D H&E stained images of mouse heart tissues from different treatment groups, with quantification of cross-sectional area of the heart tissues(scale bar: 500 μm/50 μm); E Assessment of CM size with WGA staining results (scale bar: 50 μm); F Evaluation of myocardial interstitial fibrosis deposition in mouse ventricles with Sirius Red staining (left) and Masson staining (right), where CVF (Collage Volume Fraction) quantifies the ratio of Masson-stained blue area to total heart chamber area (scale bar: 500 μm/50 μm), fibrotic area in mice indicated by picro sirius red-positive area (scale bar: 50 μm); G Western blot analysis of BNP, β-MHC, and collagen I protein expression changes in heart tissues from different treatment groups; (H) TUNEL staining detecting apoptosis in mouse myocardial tissue, with red fluorescence representing apoptotic cells and blue fluorescence from DAPI nuclear staining (scale bar: 50 μm); I Immunohistochemical staining to assess the expression of p-STAT3 protein in mouse heart tissues (scale bar: 50 μm); J Prussian blue staining for detecting iron ion content in mouse heart tissue (scale bar: 50 μm); K Detection of ROS levels in mouse heart tissues from different treatment groups; L Assessment of GSH/GSSG ratio in mouse heart tissues from different treatment groups; M Measurement of MDA content in mouse heart tissues from different treatment groups; N RT-qPCR analysis of transcription levels of ferroptosis-related genes Slc7a11 and Gpx4 in mouse heart tissues from different treatment groups; O Representative TEM images of mouse heart tissues from different treatment groups(scale bar: 2 μm). Quantitative data in the figures are presented as Mean ± SD, with 6 mice per group in each experiment, where * represents a comparison between two groups, ** indicates p < 0.01, *** indicates p < 0.001, and **** indicates p < 0.0001

Article Snippet: Subsequently, 2 mg of the STAT3 agonist Colivelin (referred to as Col, HY-P1061, MedChemExpress, USA) was added to the mixture and vigorously stirred at 30 °C.

Techniques: Staining, Western Blot, Expressing, TUNEL Assay, Fluorescence, Immunohistochemical staining, Quantitative RT-PCR, Comparison

Journal: Frontiers in Pharmacology

Article Title: Intermedin Reduces Oxidative Stress and Apoptosis in Ventilator-Induced Lung Injury via JAK2/STAT3

doi: 10.3389/fphar.2021.817874

Figure Lengend Snippet: IMD inhibited the JAK2/STAT3 pathway in VILI. Western blot analysis of p-JAK2, JAK2, p-STAT3, STAT3 and β-actin in lung tissues (A) and ECs (B) . * p < 0.05.

Article Snippet: In some experiments, the JAK2/STAT3 agonist Colivelin TFA (MedChemExpress, Shanghai, China, 1 mg/kg) was injected intraperitoneally into the mice 30 min before the IMD1-53 injection.

Techniques: Western Blot

Journal: Frontiers in Pharmacology

Article Title: Intermedin Reduces Oxidative Stress and Apoptosis in Ventilator-Induced Lung Injury via JAK2/STAT3

doi: 10.3389/fphar.2021.817874

Figure Lengend Snippet: JAK2/STAT3 pathway was involved in IMD-mediated protection. (A) Western blot analysis of p-JAK2, JAK2, p-STAT3, STAT3 and β-actin in lung tissues. (B) Cell apoptosis rate was detected using flow cytometry. (C) Cell ROS was detected using flow cytometry. * p < 0.05.

Article Snippet: In some experiments, the JAK2/STAT3 agonist Colivelin TFA (MedChemExpress, Shanghai, China, 1 mg/kg) was injected intraperitoneally into the mice 30 min before the IMD1-53 injection.

Techniques: Western Blot, Flow Cytometry

Journal: Open Medicine

Article Title: Shikonin alleviates asthma phenotypes in mice via an airway epithelial STAT3-dependent mechanism

doi: 10.1515/med-2024-1016

Figure Lengend Snippet: Shikonin suppresses the expression and localization of p-STAT3 in airway epithelial cells. (a) Western blot analysis was conducted to examine the phosphorylation level of STAT3 in mouse airway epithelial cells. (b) The expression pattern of p-STAT3 in airway epithelial cells was detected by immunofluorescence staining. Compared with the control group, ** p < 0.01; compared with the OVA group, ## p < 0.01; compared with the HDM group, △△ p < 0.01, n = 3.

Article Snippet: STAT3 agonist (Colivelin) was acquired from Tocris (Bristol, Britain).

Techniques: Expressing, Western Blot, Phospho-proteomics, Immunofluorescence, Staining, Control

Journal: Open Medicine

Article Title: Shikonin alleviates asthma phenotypes in mice via an airway epithelial STAT3-dependent mechanism

doi: 10.1515/med-2024-1016

Figure Lengend Snippet: Activation of STAT3 can reverse the inhibitory effect of shikonin on the function of MLE-12 cells. (a) Western blot analysis was performed to measure the expression levels of STAT3 and p-STAT3 in mouse epithelial cell line MLE-12. (b) The localization of p-STAT3 in mouse bronchial epithelial cell line MLE-12 was detected by immunofluorescence staining. (c) Western blot was applied to measure the expression levels of HIF-α, IFN-γ, IL-1β, and MMP9. (d) ELISA was used to detect the concentrations of inflammatory factors (IL-1β and IFN-γ) in the MLE-12 cell medium. Compared with the control group, ** p < 0.01; compared with the model group, ## p < 0.01; compared with the shikonin group, △ p < 0.05; compared with the shikonin group, △△ p < 0.01, n = 3.

Article Snippet: STAT3 agonist (Colivelin) was acquired from Tocris (Bristol, Britain).

Techniques: Activation Assay, Western Blot, Expressing, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Control

Journal: Bioengineered

Article Title: Long noncoding RNA regulatory factor X3- antisense RNA 1 promotes non-small cell lung cancer via the microRNA-577/signal transducer and activator of transcription 3 axis

doi: 10.1080/21655979.2022.2054910

Figure Lengend Snippet: The primer sequence in PCR

Article Snippet: In addition, the cells were treated with the STAT3 agonist colivelin (Cat.No. sc-361,153, Santa Cruz Biotechnology, California, USA) as required.

Techniques: Sequencing

Journal: Bioengineered

Article Title: Long noncoding RNA regulatory factor X3- antisense RNA 1 promotes non-small cell lung cancer via the microRNA-577/signal transducer and activator of transcription 3 axis

doi: 10.1080/21655979.2022.2054910

Figure Lengend Snippet: RFX3-AS1 targeted miR-577 to regulate STAT3. A: Starbase database was adopted for analysis of base pairing between miR-577 and STAT3. B: Dual-luciferase reporter assay was performed in 293 T cells transfected with STAT3-WT or STAT3-MT, with miR-NC or miR-577 mimics. C-D: The p-STAT3/STAT3 profile after overexpression of RFX3-AS1 or transfection of miR-577 mimics was monitored by WB. E. IHC was conducted for detecting p-STAT3 in the tumor tissues shown in . NS P > 0.05, ** P < 0.01, *** P < 0.001(vs.miR-NC or NC group). N = 3.

Article Snippet: In addition, the cells were treated with the STAT3 agonist colivelin (Cat.No. sc-361,153, Santa Cruz Biotechnology, California, USA) as required.

Techniques: Luciferase, Reporter Assay, Transfection, Over Expression

Journal: Bioengineered

Article Title: Long noncoding RNA regulatory factor X3- antisense RNA 1 promotes non-small cell lung cancer via the microRNA-577/signal transducer and activator of transcription 3 axis

doi: 10.1080/21655979.2022.2054910

Figure Lengend Snippet: Activating STAT3 weakened the tumor-suppressive effect of miR-577 in NSCLC. PC-9 cells were transfected with miR-577 mimics or interfered with colivelin (50 µg/mL) for 12 hours. A: WB was performed to verify the STAT3 expression after each factor treatment. B: CCK-8 was utilized to testify cell proliferation. C: Cell viability was analyzed by the cell colony formation assay. D. Cell apoptosis was analyzed by FCM. E-F: Transwell assay was implemented to monitor cell migration and invasion. G: Protein expression of E-cadherin, Vimentin and N-cadherin was examined by WB. The protein profiles of Bcl2, Bax and Caspase3 were compared by WB. * P < 0.5, ** P < 0.01, *** P < 0.001 (vs.miR-NC group); # P < 0.5, ## P < 0.01, ### P < 0.001(vs.miR-577 group) N = 3.

Article Snippet: In addition, the cells were treated with the STAT3 agonist colivelin (Cat.No. sc-361,153, Santa Cruz Biotechnology, California, USA) as required.

Techniques: Transfection, Expressing, CCK-8 Assay, Colony Assay, Transwell Assay, Migration

Journal: Bioengineered

Article Title: Long noncoding RNA regulatory factor X3- antisense RNA 1 promotes non-small cell lung cancer via the microRNA-577/signal transducer and activator of transcription 3 axis

doi: 10.1080/21655979.2022.2054910

Figure Lengend Snippet: Graphical abstract. Overexpression of RFX3-AS1 targets and inhibits miR-577 expression, thereby activating STAT3, which promotes NSCLC cell proliferation, viability, migration, invasion, and EMT, and dampens apoptosis.

Article Snippet: In addition, the cells were treated with the STAT3 agonist colivelin (Cat.No. sc-361,153, Santa Cruz Biotechnology, California, USA) as required.

Techniques: Over Expression, Expressing, Migration